Development of a 16S rRNA-gen-based primersystem for the detection of saccharopolyspora rectivirgula in bioaerosols

  • Project number : F 2042
  • Institution : Institut für Angewandte Mikrobiologie (IFZ)
  • Status : Completed Project

Description :

Saccharopolyspora rectivirgula is a causative organism of hypersensitive pneumonitis. The cultivation based detection of this bacterial strain is very difficult because the phenotypic characteristica, depending on the cultivation conditions are highly variable. Therefore a strain specific pirmer system should be generated to gain a gen based detection system allowing the rapid and specific detection of Saccharopolyspora rectivirgula with a quantitative real-time PCR in bioaerosols. Each analystical step of this method should be checked thoroughly.

Contact

Unit 4.II.2 "Bioaerosols"

Phone: +49 231 9071-1971
Fax: +49 231 9071-2070