One of the biggest occupational risks of people employed in industrial livestock farming can be found in the exposure to airborne microorganisms as a part of livestock associated bioaerosols. In this context Staphylococcus aureus is becoming increasingly important. So far, a quick quantification of bacterial genera or species based on cultivation-dependent methods is time-consuming and staff-intensive because the consisting cultivation-based methods generally require an isolation and a confirmation (identification) of the target bacteria that have been expanded within the quantification process. In the course of the work presented here a highly specific and sensitive PCR-based system (PCR: polymerase chain reaction) was established to detect exposures to S. aureus and to accomplish an improvement in the individual-related prevention of diseases caused by biological agents.
Please download the article "Establishment of a PCR-based detection system of Staphylococcus aureus in bioaerosol samples of pig fattening units" (in German only).
S. Eichberg, U. Jäckel:
Etablierung eines PCR-basierten Nachweissystems für den Nachweis von Staphylococcus aureus in Bioaerosolproben aus Schweinemastanlagen.
in: Gefahrstoffe - Reinhaltung der Luft 76(2016) Issue 7-8 2016. pages 267-274, PDF file
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