Detection of airborne biological agents under difficult analytical conditions - background measurement and exclusion of inhibitory substances
The project establishes new measurement methods for the molecular-biological analysis of biological background levels that will allow for exposure assessment even under difficult conditions (low load situations, presence of inhibitory substances). Therefore, the precipitation of microorganisms from large air volumes by stationary collection systems is tested, as well as the quantification of the collected genomic material using digital-PCR. The methodology developed will be tested under field conditions and can make an important contribution to the improvement of workplace measurements and the interpretation of background load.
Unit 4.7 "Biological Agents"