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Development of a 16S rRNA-gen based primersystem for the detection of Thermoactinomyces species in bioaerosols

Project number: F 2215 Institution: Justus-Liebig Universität Gießen - Institut für Angewandte Mikrobiologie - IFZ Status: Completed Project

Description:

Thermoactinomyces vulgaris is a causative organism of hypersensitive pneumonitis. The cultivation based detection of this bacterial strain is very difficult because the phenotypic characteristica, depending on the cultivation conditions are highly variable. Therefore a strain specific primer system should be generated to gain a gen based detection system allowing the rapid and specific detection of Thermoactinomyces vulgaris with a quantitative real-time PCR in bioaerosols. Each analystical step of this method should be ckecked thoroughly.

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