The cultivation based detection of bacterial strains causing respiratory problems (e.g. hypersensitive pneumonitis) in complex bioaerosols is very difficult because the phenotypic characteristica e.g. depending on the cultivation conditions are highly variable. Therefore strain specific primer systems known from the literature should be tested to detect and quantify relevant bacterial species in bioaerosols at work places. Each analystical step of this method should be checked thoroughly.
Unit 4.7 "Biological Agents"